Okazaki M, Morita K, Kochi N, Araki K, Yoshizawa M, Wada H, Shibata M, Watanabe N, Egami T, Furuya N, Kanamori M, Shimazaki S, Uchimura H
Department of Clinical Laboratories, Kyorin University Hospital.
Kansenshogaku Zasshi. 1998 Jul;72(7):688-93. doi: 10.11150/kansenshogakuzasshi1970.72.688.
Strains of Burkholderia cepacia isolated in our hospital from November 1995 to September 1996 were classified with randomly amplified polymorphic DNA-PCR (RAPD-PCR) and conventional biochemical tests (ID test.NF-18, API20NE, and Neg Combo 4J kit), and intrahospital isolates of B. cepacia were analysed. During the period 28 strains from inpatients and 2 from medical apparatus were isolated. Twenty four of 28 (85.7%) were from sputum. In 1996 from January to February, 20 strains were detected from 8 inpatients, and two strains were from the nebulizers at the Trauma and Critical Care Center (TCC). With typing of B. cepacia by conventional methods no epidemiological relations among isolates were found. However, DNA patterns of original isolates from the nebulizers at TCC by RAPD-PCR were identical with those of isolates in sputa from patients in other wards who had stayed at TCC, indicating that TCC was an initial source of transmission and the strain was transmitted with the patients to the wards. These results suggest that RAPD-PCR method might be a useful tool to analyse an epidemiological survey for intrahospital transmission of isolate.
1995年11月至1996年9月期间，我院分离出的洋葱伯克霍尔德菌菌株采用随机扩增多态性DNA - PCR（RAPD - PCR）和传统生化试验（ID试验、NF - 18、API20NE和Neg Combo 4J试剂盒）进行分类，并对院内分离的洋葱伯克霍尔德菌进行分析。在此期间，从住院患者中分离出28株，从医疗设备中分离出2株。28株中的24株（85.7%）来自痰液。1996年1月至2月，从8名住院患者中检测出20株，另外2株来自创伤与重症监护中心（TCC）的雾化器。采用传统方法对洋葱伯克霍尔德菌进行分型时，未发现分离株之间存在流行病学关联。然而，通过RAPD - PCR对TCC雾化器原始分离株的DNA图谱分析发现，其与在TCC住院过的其他病房患者痰液中的分离株相同，这表明TCC是初始传播源，该菌株随患者传播至各病房。这些结果表明，RAPD - PCR方法可能是分析院内分离株传播流行病学调查的有用工具。
