Nadala E C, Tapay L M, Loh P C
Department of Microbiology, University of Hawaii, Honolulu 96822, USA.
Dis Aquat Organ. 1998 Jul 30;33(3):221-9. doi: 10.3354/dao033221.
A non-occluded baculovirus-like agent recently isolated by this laboratory from moribund Penaeus japonicus shrimps obtained from China and named Chinese baculovirus (CBV) was purified and some of its properties characterized. Under the electron microscope, negatively stained virus particles were rod-shaped, enveloped, and measured 322 to 378 nm in length and 130 to 159 nm in diameter. The nucleoprotein core exhibited a unique striated structure and measured 316 to 350 nm in length and 65 to 66 nm in diameter. The striations appear to be the result of the stacking of ring-like structures. These rings consisted of 2 rows of 12 to 14 globular subunits. Each globular subunit measured approximately 10 nm in diameter. SDS-PAGE gels of purified virus preparations showed, among several, 4 prominent protein bands with approximate molecular weights of 19, 23.5, 27.5 and 75 kDa. The structural viral proteins were identified by western blot analysis using polyclonal hyperimmune serum made against purified CBV. The 19, 27.5, and 75 kDa structural proteins were determined to be non-glycosylated components associated with the viral envelope. The 23.5 kDa protein, also non-glycosylated, was identified with the capsid structure. Viral genomic DNA digested with Hind III restriction endonuclease revealed at least 29 different fragments with a conservatively estimated total size of at least 183 kb.
本实验室最近从中国获得的濒死日本对虾中分离出一种非封闭型杆状病毒样病原体，并将其命名为中国杆状病毒（CBV），对其进行了纯化并表征了部分特性。在电子显微镜下，经负染的病毒粒子呈杆状，有包膜，长度为322至378纳米，直径为130至159纳米。核蛋白核心呈现出独特的条纹状结构，长度为316至350纳米，直径为65至66纳米。这些条纹似乎是环状结构堆叠的结果。这些环由2排12至14个球状亚基组成。每个球状亚基直径约为10纳米。纯化病毒制剂的SDS-PAGE凝胶显示，在几条带中，有4条明显的蛋白带，其近似分子量分别为19、23.5、27.5和75 kDa。使用针对纯化CBV制备的多克隆超免疫血清通过蛋白质印迹分析鉴定了病毒结构蛋白。确定19、27.5和75 kDa的结构蛋白是与病毒包膜相关的非糖基化成分。23.5 kDa的蛋白也是非糖基化的，与衣壳结构相关。用Hind III限制性内切酶消化的病毒基因组DNA显示至少有29个不同的片段，保守估计总大小至少为183 kb。
