Heil W, Grunewald R, Amend M, Heins M
Zentrallaboratorium, Klinikum Wuppertal GmbH, Universität Witten/Herdecke, Germany.
Clin Chem Lab Med. 1998 Jun;36(7):459-62. doi: 10.1515/CCLM.1998.077.
There is no comprehensive study on the stability of coagulation analytes in plasma. We therefore determined the influence of storage time and temperature on prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, factors V and VIII, antithrombin III, protein C and S in plasma from 20 healthy subjects and 20 patients receiving heparin therapy. The stability in plasma, defined as the period during which there was a change of less than 10% from the initial value, was 8 hours for activated partial thromboplastin time, 24 hours for prothrombin time, 48 hours for factor V and 7 days for thrombin time, fibrinogen, protein C and antithrombin III in healthy subjects at 6 degrees C. Factor VIII and protein S showed 19 and 12 % reduction in activity, respectively, after 8 hours. In volunteers not treated with heparin therapy, activated partial thromboplastin time was stable for 8 hours; prothrombin time for 48 hours; and thrombin time, fibrinogen and antithrombin III for 7 days with sample storage at room temperature. Factor VIII showed a decrease of 18 % after 8 hours. For patients receiving heparin therapy, the stability of the analytes in plasma stored at 6 degrees C was 8 hours for thrombin time, 24 hours for prothrombin time and activated partial thromboplastin time and 7 days for fibrinogen and antithrombin III. Factors V and VIII showed a decrease of 13 % and 20 % respectively after 8 hours. When the plasma of these patients was stored at room temperature, factor V was stable for 8 hours, and prothrombin time for 24 hours, whereas fibrinogen and antithrombin III remained unchanged for 7 days. Activated partial thromboplastin time showed an increase of 13 %, thrombin time a fall of 16 %, and factor VIII a decrease of 18 % after 8 hours.
目前尚无关于血浆中凝血分析物稳定性的全面研究。因此，我们测定了储存时间和温度对20名健康受试者以及20名接受肝素治疗患者血浆中凝血酶原时间、活化部分凝血活酶时间、凝血酶时间、纤维蛋白原、因子V和VIII、抗凝血酶III、蛋白C和S的影响。在6摄氏度下，健康受试者血浆中活化部分凝血活酶时间的稳定性（定义为与初始值相比变化小于10%的时间段）为8小时，凝血酶原时间为24小时，因子V为48小时，凝血酶时间、纤维蛋白原、蛋白C和抗凝血酶III为7天。8小时后，因子VIII和蛋白S的活性分别降低了19%和12%。在未接受肝素治疗的志愿者中，活化部分凝血活酶时间在室温下储存8小时稳定；凝血酶原时间48小时稳定；凝血酶时间、纤维蛋白原和抗凝血酶III 7天稳定。8小时后，因子VIII降低了18%。对于接受肝素治疗的患者，6摄氏度下储存的血浆中，凝血酶时间的稳定性为8小时，凝血酶原时间和活化部分凝血活酶时间为24小时，纤维蛋白原和抗凝血酶III为7天。8小时后，因子V和VIII分别降低了13%和20%。当这些患者的血浆在室温下储存时，因子V 8小时稳定，凝血酶原时间24小时稳定，而纤维蛋白原和抗凝血酶III 7天不变。8小时后，活化部分凝血活酶时间增加了13%，凝血酶时间下降了16%，因子VIII降低了18%。
