犬类的蛋白C活性：一种商业化人类比色法的适配以及储存时间和温度影响的评估
Protein C activity in dogs: adaptation of a commercial human colorimetric assay and evaluation of effects of storage time and temperature.
作者信息
Fry Michael M, Snyder Karl R, Tobias Karen M, Williamson Baye G, Reed G Ann
机构信息
Department of Pathobiology, College of Veterinary Medicine, The University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
出版信息
Vet Med Int. 2011;2011:751849. doi: 10.4061/2011/751849. Epub 2011 May 30.
Objectives of this study were to adapt a commercial human protein C (PC) colorimetric assay for use in dogs and to investigate effects of various storage conditions. The human assay was modified by using pooled canine plasma for calibration and by increasing the activation time. PC activity was measured in fresh canine plasma and in plasma stored under various conditions. PC activity of some stored samples was significantly different from that of fresh plasma; however, differences were small. No difference was detected in samples stored under similar conditions but analyzed in different laboratories using similar methodology. Results of this study indicate that the human colorimetric assay is suitable for canine samples if pooled canine plasma is used for calibration, that Clinical and Laboratory Standards Institute sample storage guidelines developed for testing in humans are appropriate for dogs, and that comparisons of results from laboratories using similar methodology are legitimate.
本研究的目的是调整一种商业化的人蛋白C（PC）比色测定法以用于犬类，并研究各种储存条件的影响。通过使用混合犬血浆进行校准并延长激活时间，对人用测定法进行了修改。在新鲜犬血浆和在各种条件下储存的血浆中测量PC活性。一些储存样本的PC活性与新鲜血浆的活性有显著差异；然而，差异很小。在相似条件下储存但在不同实验室使用相似方法进行分析的样本中未检测到差异。本研究结果表明，如果使用混合犬血浆进行校准，人比色测定法适用于犬类样本，为人类检测制定的临床和实验室标准协会样本储存指南适用于犬类，并且使用相似方法的实验室之间的结果比较是合理的。
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