Jansen H J, van der Hoeven J S, van den Kieboom C W, Göertz J H, Camp P J, Bakkeren J A
Department of Periodontology and Preventive Dentistry, University of Nijmegen, the Netherlands.
Oral Microbiol Immunol. 1994 Dec;9(6):345-51. doi: 10.1111/j.1399-302x.1994.tb00284.x.
Several subgingival microorganisms were tested for their ability to utilize human immunoglobulin G (IgG) as a substrate for growth. This was done using a protein-free chemically defined medium, supplemented with IgG. Stimulation of growth was observed for Capnocytophaga ochracea, Porphyromonas asaccharolytica, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Prevotella oralis, Lactobacillus catenaforme and Streptococcus intermedius. Immunoelectrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a protein assay demonstrated that P. intermedia and P. endodontalis completely degraded the protein chains of IgG. Partial breakdown of IgG was observed for P. asaccharolytica and C. ochracea, whereas P. oralis cleaved the IgG heavy chain, yielding Fc and Fab fragments. All these bacteria utilized IgG as a substrate for growth. Binding studies using an enzyme-linked immunosorbent assay, revealed complete loss of in vitro antigen-antibody binding capacity after incubation of specific IgG with P. endodontalis and partial loss of binding with P. intermedia, P. gingivalis, C. ochracea or Fusobacterium nucleatum. Degradation or inactivation of IgG by oral bacteria is thought to be important in the causation of polymicrobial infections.
对几种龈下微生物利用人免疫球蛋白G（IgG）作为生长底物的能力进行了测试。这是通过使用不含蛋白质的化学限定培养基并添加IgG来完成的。观察到以下微生物在该培养基中生长受到刺激：黄褐二氧化碳嗜纤维菌、不解糖卟啉单胞菌、牙髓卟啉单胞菌、牙龈卟啉单胞菌、中间普氏菌、口腔普氏菌、链状乳杆菌和中间链球菌。免疫电泳、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质测定表明，中间普氏菌和牙髓卟啉单胞菌完全降解了IgG的蛋白链。观察到不解糖卟啉单胞菌和黄褐二氧化碳嗜纤维菌对IgG有部分降解，而口腔普氏菌切割了IgG重链，产生Fc和Fab片段。所有这些细菌都利用IgG作为生长底物。使用酶联免疫吸附测定进行的结合研究表明，特异性IgG与牙髓卟啉单胞菌孵育后，体外抗原-抗体结合能力完全丧失，与中间普氏菌、牙龈卟啉单胞菌、黄褐二氧化碳嗜纤维菌或具核梭杆菌孵育后，结合能力部分丧失。口腔细菌对IgG的降解或失活被认为在多微生物感染的病因学中起重要作用。
