Parrilla Inmaculada, Martinez Cristina A, Cambra Josep M, Lucas Xiomara, Ferreira-Dias Graça, Rodriguez-Martinez Heriberto, Cuello Cristina, Gil Maria A, Martinez Emilio A
Faculty of Veterinary Medicine, International Excellence Campus for Higher Education and Research "Campus Mare Nostrum", University of Murcia, 30071 Murcia, Spain.
Campus de Ciencias de la Salud, Institute for Biomedical Research of Murcia (IMIB-Arrixaca), 30071 Murcia, Spain.
Animals (Basel). 2020 Nov 4;10(11):2028. doi: 10.3390/ani10112028.
In the context of porcine embryo transfer (ET) technology, understanding the tightly regulated local uterine immune environment is crucial to achieve an adequate interaction between the transferred embryos and the receiving endometrium. However, information is limited on the uterine immune status of cyclic-recipient sows when receiving embryos during ET. The present study postulated that the anti- and proinflammatory cytokine profile 6 days after the onset of estrus differs between endometria from uninseminated cyclic sows and blastocyst-bearing sows. On Day 6 of the cycle, endometrial explants were collected from sows inseminated or not inseminated during the postweaning estrus and cultured for 22 h. The culture medium was then analyzed for the contents of a total of 16 cytokines using Luminex MAP technology. The results showed important differences in the endometrial production of most cytokines between the sow categories, with a predominant anti-inflammatory environment displayed by the blastocyst-bearing endometria. These findings suggest that sperm, seminal plasma (SP) and/or early embryos modify the uterine environment by inducing an immune-tolerant cytokine profile already visible at Day 6. Whether the SP or some of its active components may help to develop strategies to maximize the reproductive performance of recipients after ET needs further investigation.
在猪胚胎移植（ET）技术背景下，了解严格调控的局部子宫免疫环境对于实现移植胚胎与受体子宫内膜之间的充分相互作用至关重要。然而，关于在胚胎移植期间接受胚胎的周期性受体母猪的子宫免疫状态的信息有限。本研究推测，发情开始后6天，未授精的周期性母猪和怀有囊胚的母猪的子宫内膜中抗炎和促炎细胞因子谱存在差异。在周期的第6天，从断奶后发情期授精或未授精的母猪收集子宫内膜外植体，并培养22小时。然后使用Luminex MAP技术分析培养基中总共16种细胞因子的含量。结果显示，不同类别母猪的子宫内膜中大多数细胞因子的产生存在重要差异，怀有囊胚的子宫内膜呈现出主要的抗炎环境。这些发现表明，精子、精浆（SP）和/或早期胚胎通过诱导在第6天就已可见的免疫耐受细胞因子谱来改变子宫环境。SP或其某些活性成分是否有助于制定策略以最大化胚胎移植后受体的繁殖性能，还有待进一步研究。
