[婴儿未成熟血管瘤中未检测到HHV-8病毒]
[Absence of HHV-8 virus detected in immature hemangiomas in infants].
作者信息
Dupin N, Enjolras O, Wassef M, Gorin I, Gradadam M, Huraux J M, Agut H, Escande J P
机构信息
Service de Dermato-Vénéréologie, Hôpital Tarnier-Cochin, Paris.
出版信息
Ann Dermatol Venereol. 1998 Feb;125(2):98-9.
INTRODUCTION
The aim of our study was to search for the presence of HHV-8 DNA sequences in Biopsy specimens from hemangioma of the infancy.
MATERIAL AND METHODS
The study included 9 biopsies from hemangioma. DNA of human beta-globin gene and HHV-8 were searched for by PCR using specific primers. Amplified products were revealed after an hybridization with an internal probe digoxigenin-labelled.
RESULTS
Human beta-globin gene could be detected in all samples illustrating the absence of PCR inhibitor. HHV-8 could never be detected in samples analyzed.
DISCUSSION
Our study does not imply any causative role of HHV-8 in the pathogenesis of hemangioma. This result must be confirmed by serologic studies.
引言
我们研究的目的是在婴儿血管瘤活检标本中寻找人类疱疹病毒8型（HHV-8）DNA序列。
材料与方法
该研究包括9例血管瘤活检标本。使用特异性引物通过聚合酶链反应（PCR）检测人类β-珠蛋白基因和HHV-8的DNA。扩增产物在与地高辛标记的内部探针杂交后显现出来。
结果
在所有样本中均可检测到人类β-珠蛋白基因，表明不存在PCR抑制剂。在所分析的样本中从未检测到HHV-8。
讨论
我们的研究并不意味着HHV-8在血管瘤发病机制中具有任何致病作用。这一结果必须通过血清学研究加以证实。
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