电子显微镜下连续切片的计算机三维重建

Computerized three-dimensional reconstructions of serial sections in electron microscopy.

作者信息

Kriete A, Magdowski G

机构信息

Institute of Anatomy and Cytobiology, Justus-Liebig-University, Giessen, Fed. Rep. of Germany.

出版信息

Ultramicroscopy. 1990 Jan;32(1):48-54. doi: 10.1016/0304-3991(90)90093-2.

DOI:10.1016/0304-3991(90)90093-2

PMID:2336733

Abstract

To improve reconstructive 3D electron microscopy novel methods are discussed to represent and process serial section images in a cuberille environment. This includes the analysis of the transfer characteristics of the image detection system, the use of laser-induced fiducials for deformation correction and alignment, the control of section thickness by EELS and the use of ESI to image thick sections.

摘要

为了改进三维重建电子显微镜技术，本文讨论了在立方环境中表示和处理连续切片图像的新方法。这包括分析图像检测系统的传输特性、使用激光诱导基准点进行变形校正和对齐、通过电子能量损失谱（EELS）控制切片厚度以及使用电子扫描成像（ESI）对厚切片进行成像。