Costa P, Bahrman N, Frigerio J M, Kremer A, Plomion C
INRA, Laboratoire de Génétique et Amélioration des Arbres Forestiers, Cestas, France.
Plant Mol Biol. 1998 Nov 1;38(4):587-96. doi: 10.1023/a:1006006132120.
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) was used to identify drought-responsive proteins during progressive water deprivation of two-year old maritime pine seedlings. Stress was applied by withholding water during vegetative growth. Needles were sampled before, during and after the stress. Out of about 1000 spots that were quantified by computer analysis, 38 responded during stress. Some proteins were accumulated while others were suppressed. One to three internal microsequences were obtained for 11 proteins, 10 of which were identified on the basis of sequence homologies. These proteins are quite diverse and are involved in photosynthesis, cell elongation, antioxidant metabolism and lignification.
二维聚丙烯酰胺凝胶电泳（2D-PAGE）用于鉴定两岁欧洲赤松幼苗在渐进性水分剥夺过程中的干旱响应蛋白。在营养生长期间通过停水施加胁迫。在胁迫前、胁迫期间和胁迫后采集针叶样本。通过计算机分析定量的约1000个斑点中，有38个在胁迫期间有响应。一些蛋白质积累，而另一些则受到抑制。为11种蛋白质获得了1至3个内部微序列，其中10种基于序列同源性得到鉴定。这些蛋白质种类繁多，参与光合作用、细胞伸长、抗氧化代谢和木质化。
