Chan W C, Nie S
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
Science. 1998 Sep 25;281(5385):2016-8. doi: 10.1126/science.281.5385.2016.
Highly luminescent semiconductor quantum dots (zinc sulfide-capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible. Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.
高发光半导体量子点（硫化锌包覆的硒化镉）已与生物分子共价偶联，用于超灵敏生物检测。与若丹明等有机染料相比，这类发光标记物的亮度高20倍，抗光漂白稳定性高100倍，谱线宽度窄三分之一。这些纳米尺寸的缀合物具有水溶性且生物相容性良好。用转铁蛋白标记的量子点在培养的HeLa细胞中经历了受体介导的内吞作用，而用免疫分子标记的量子点则能识别特异性抗体或抗原。
