Oe Takuma, Dechojarassri Duangkamol, Kakinoki Sachiro, Kawasaki Hideya, Furuike Tetsuya, Tamura Hiroshi
Faculty of Chemistry, Materials and Bioengineering, Kansai University, Osaka 564-8680, Japan.
Organization for Research and Development of Innovative Science and Technology (ORDIST), Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
J Funct Biomater. 2023 Apr 4;14(4):199. doi: 10.3390/jfb14040199.
Herein, improving the antibacterial activity of a hydrogel system of sodium alginate (SA) and basic chitosan (CS) using sodium hydrogen carbonate by adding AgNPs was investigated. SA-coated AgNPs produced by ascorbic acid or microwave heating were evaluated for their antimicrobial activity. Unlike ascorbic acid, the microwave-assisted method produced uniform and stable SA-AgNPs with an optimal reaction time of 8 min. Transmission electron microscopy (TEM) confirmed the formation of SA-AgNPs with an average particle size of 9 ± 2 nm. Moreover, UV-vis spectroscopy confirmed the optimal conditions for SA-AgNP synthesis (0.5% SA, 50 mM AgNO, and pH 9 at 80 °C). Fourier transform infrared (FTIR) spectroscopy confirmed that the -COO group of SA electrostatically interacted with either the Ag or -NH of CS. Adding glucono-δ-lactone (GDL) to the mixture of SA-AgNPs/CS resulted in a low pH (below the pa of CS). An SA-AgNPs/CS gel was formed successfully and retained its shape. This hydrogel exhibited 25 ± 2 mm and 21 ± 1 mm inhibition zones against and and showed low cytotoxicity. Additionally, the SA-AgNP/CS gel showed higher mechanical strength than SA/CS gels, possibly due to the higher crosslink density. In this work, a novel antibacterial hydrogel system was synthesized via 8 min of microwave heating.
在此，研究了通过添加AgNPs使用碳酸氢钠来提高海藻酸钠（SA）和碱性壳聚糖（CS）水凝胶系统的抗菌活性。评估了通过抗坏血酸或微波加热制备的SA包覆的AgNPs的抗菌活性。与抗坏血酸不同，微波辅助方法产生了均匀且稳定的SA-AgNPs，最佳反应时间为8分钟。透射电子显微镜（TEM）证实形成了平均粒径为9±2nm的SA-AgNPs。此外，紫外可见光谱证实了SA-AgNP合成的最佳条件（0.5%SA、50mM AgNO₃和80℃下pH值为9）。傅里叶变换红外（FTIR）光谱证实SA的-COO基团与CS的Ag或-NH发生静电相互作用。向SA-AgNPs/CS混合物中添加葡萄糖酸-δ-内酯（GDL）导致低pH值（低于CS的pKa）。成功形成了SA-AgNPs/CS凝胶并保持其形状。这种水凝胶对[具体菌种1]和[具体菌种2]表现出25±2mm和21±1mm的抑菌圈，且细胞毒性较低。此外，SA-AgNP/CS凝胶显示出比SA/CS凝胶更高的机械强度，这可能归因于更高的交联密度。在这项工作中，通过8分钟的微波加热合成了一种新型抗菌水凝胶系统。
