Hurwitz E, Maron R, Arnon R, Sela M
Cancer Biochem Biophys. 1976 May;1(4):197-202.
Fab dimers were prepared by pepsin digestion from the immunoglobulin fraction of a rabbit antiserum towards the Yac Moloney virus lymphoma cells. Daunomycin was attatched to these (Fab)2 by covalent binding. The resultant conjugates exerted pharmacological toxic activity and specificity towards Yac target cells similar to that observed previously with conjugates of intact anti-Yac IgG. The activity was manifested both in vitro by inhibition of RNA synthesis and by the effect of reduction of the growth of the tumor cells in vivo after short exposure to the conjugates. The potential advantage of using an IgG molecule devoid of its Fc portion is discussed.
通过用胃蛋白酶消化兔抗Yac莫洛尼病毒淋巴瘤细胞抗血清的免疫球蛋白部分制备Fab二聚体。柔红霉素通过共价结合附着于这些（Fab）2上。所得结合物对Yac靶细胞表现出与先前观察到的完整抗Yac IgG结合物相似的药理毒性活性和特异性。该活性在体外通过抑制RNA合成以及在体内短时间接触结合物后对肿瘤细胞生长的抑制作用得以体现。文中讨论了使用不含Fc部分的IgG分子的潜在优势。
