Levy R, Hurwitz E, Maron R, Arnon R, Sela M
Cancer Res. 1975 May;35(5):1182-6.
Daunomycin was covalently bound to immunoglobulins by periodate oxidation as described in the preceding paper. Conjugates were prepared with immunoglobulins directed against either of two mouse lymphoid tumors or with nonspecific immunoglobulins. These conjugates were tested for their toxic effects on various tumor target cells as measured either by their inhibition of RNA synthesis or by their reduction of the growth of the tumor cells after transplantation. We found that the drug preferentially affected the target cells that the antibody to which it was attached could recognize. These daunomycin-antibody conjugates are therefore sufficiently toxic and selective in their effects to be potentially useful in in vivo therapeutic studies.
如前文所述，通过高碘酸盐氧化将柔红霉素与免疫球蛋白共价结合。用针对两种小鼠淋巴瘤之一的免疫球蛋白或非特异性免疫球蛋白制备偶联物。通过抑制RNA合成或移植后肿瘤细胞生长的减少来测量，测试这些偶联物对各种肿瘤靶细胞的毒性作用。我们发现，该药物优先影响其附着的抗体能够识别的靶细胞。因此，这些柔红霉素-抗体偶联物具有足够的毒性和选择性，在体内治疗研究中可能有用。
