Nilsson J, Svensson B, Ekelund K, Christiansson A
Swedish Dairies' Association, Research and Development Department, Lund, Sweden.
Lett Appl Microbiol. 1998 Sep;27(3):168-72. doi: 10.1046/j.1472-765x.1998.00402.x.
A robust RAPD-PCR procedure for large-scale typing of Bacillus cereus was developed. It is based on a simple DNA preparation, involving only freezing and boiling of cells in water with active carbon. By using a computerized system for data collection and processing, an efficient system for handling RAPD patterns for large-scale investigations was achieved. The procedure was highly discriminatory for Bacillus cereus strains and was found to give reproducible classification of RAPD fingerprints for five reference strains.
