一种快速检测蜡样芽胞杆菌的RAPD-PCR 方法。
A RAPD-PCR method for the rapid detection of Bacillus cereus.
机构信息
Division of Applied Life Science (BK21), Graduate School, Gyeongsang National University, Jinju, Korea.
出版信息
J Microbiol Biotechnol. 2011 Mar;21(3):274-6.
Distinction of Bacillus cereus from other closely related bacilli is challenging and new efficient methods are continually demanded. From our previous work on RAPD profiles of bacilli, we found a possibility that B. cereus strains could be distinguished from other bacilli. In this work, RAPD-PCR profiles of B. cereus strains were obtained using a 10-mer (S30) as a primer, and a B. cereus specific 0.91-kb band was produced from all tested strains. The RAPD-PCR procedure also successfully detected B. cereus from spiked cheonggukjang when B. cereus cells were present at more than 10(2)/g sample.
从其他密切相关的芽孢杆菌中区分出蜡样芽孢杆菌具有挑战性，因此需要不断开发新的有效方法。从我们之前对芽孢杆菌 RAPD 图谱的研究中，我们发现有可能将蜡样芽孢杆菌菌株与其他芽孢杆菌区分开来。在这项工作中，使用 10 个碱基（S30）作为引物获得了蜡样芽孢杆菌菌株的 RAPD-PCR 图谱，并且所有测试的菌株都产生了一个特定于蜡样芽孢杆菌的 0.91kb 带。当样品中存在的蜡样芽孢杆菌细胞数超过 10（2）/g 时，RAPD-PCR 程序也成功地从污染的朝鲜泡菜中检测到了蜡样芽孢杆菌。
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