Kuwana Ritsuko, Imamura Daisuke, Takamatsu Hiromu, Watabe Kazuhito
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Biocontrol Sci. 2012 Jun;17(2):83-6. doi: 10.4265/bio.17.83.
We tried to discriminate 16 strains of the Bacillus cereus group including B. cereus, B. thuringiensis, B. mycoides, B. pseudomycoides, and B. weihenstephanensis strains by the pattern analysis of Random Amplified Polymorphic DNA (RAPD) -PCR. Eight oligonucleotides primers were prepared and the polymorphic patterns of the DNA of each strain were compared with those of others. The primers E and F gave different patterns of RAPD-PCR products in all strains of the B. cereus group, so these primers are effective tools for the discrimination of closely related strains. All eight primers showed different polymorphic patterns of DNA for the four strains of B. cereus isolated from the kitchen of a private home, which verifies the advantage of the RAPD-PCR analysis for the discrimination of isolated strains of B. cereus from the environment.
我们试图通过随机扩增多态性DNA（RAPD）-PCR的图谱分析来鉴别蜡样芽孢杆菌群的16个菌株，包括蜡样芽孢杆菌、苏云金芽孢杆菌、蕈状芽孢杆菌、假蕈状芽孢杆菌和韦氏芽孢杆菌菌株。制备了8种寡核苷酸引物，并将每个菌株DNA的多态性图谱与其他菌株进行比较。引物E和F在蜡样芽孢杆菌群的所有菌株中产生了不同的RAPD-PCR产物图谱，因此这些引物是鉴别密切相关菌株的有效工具。从一个私人住宅厨房分离出的4株蜡样芽孢杆菌，所有8种引物都显示出不同的DNA多态性图谱，这证实了RAPD-PCR分析在鉴别环境中分离出的蜡样芽孢杆菌菌株方面的优势。
