Parekh P I, Petro A E, Tiller J M, Feinglos M N, Surwit R S
Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Duke University, Durham, NC 27710, USA.
Metabolism. 1998 Sep;47(9):1089-96. doi: 10.1016/s0026-0495(98)90283-9.
We have previously shown that C57BL/6J (B6) mice develop severe obesity and diabetes if weaned onto high-fat diets, whereas A/J mice tend to be obesity and diabetes-resistant. The purpose of this study was to determine if obesity and diabetes in the B6 mouse could be completely reversed by reducing dietary fat content. After 4 months, both strains consumed more calories on a high-fat diet than on a low-fat diet, and both strains showed a higher feed efficiency (FE=weight gained/calories consumed) on the high-fat diet versus the low-fat diet. However, relative to A/J mice, B6 mice demonstrated a significantly higher FE on the high-fat diet. Hyperglycemia, hyperinsulinemia, and increased adiposity were apparent in B6 mice after 4 months on the high-fat diet regardless of whether the diet was begun at weaning or 4 months later. Correlational analyses showed that adiposity was strongly related to both insulin and glucose levels in B6 mice, but only moderately related to insulin levels in A/J mice. In obese B6 mice that were switched to a low-fat diet, obesity and diabetes were completely reversed. Adiposity, fasting glucose, and fasting insulin values in these mice were equivalent to those in B6 mice of the same age that had spent 8 months on the low-fat diet. In summary, our data show that in the B6 mouse the severity of diabetes is a direct function of obesity and diabetes is completely reversible by reducing dietary fat.
我们之前已经表明，如果将C57BL/6J（B6）小鼠断奶后喂食高脂饮食，它们会出现严重的肥胖和糖尿病，而A/J小鼠则倾向于对肥胖和糖尿病具有抗性。本研究的目的是确定通过降低饮食脂肪含量，B6小鼠的肥胖和糖尿病是否可以完全逆转。4个月后，两种品系的小鼠在高脂饮食中消耗的卡路里都比低脂饮食中多，并且两种品系在高脂饮食中的饲料效率（FE = 体重增加/消耗的卡路里）都高于低脂饮食。然而，相对于A/J小鼠，B6小鼠在高脂饮食中的FE显著更高。无论高脂饮食是在断奶时开始还是在4个月后开始，B6小鼠在高脂饮食4个月后都会出现高血糖、高胰岛素血症和肥胖增加。相关性分析表明，肥胖与B6小鼠的胰岛素和葡萄糖水平都密切相关，但与A/J小鼠的胰岛素水平仅中度相关。在改为低脂饮食的肥胖B6小鼠中，肥胖和糖尿病完全逆转。这些小鼠的肥胖、空腹血糖和空腹胰岛素值与在低脂饮食中度过8个月的同龄B6小鼠相当。总之，我们的数据表明，在B6小鼠中，糖尿病的严重程度是肥胖的直接函数，并且通过降低饮食脂肪，糖尿病是完全可逆的。
