Radlwimmer F B, Yokoyama S
Department of Biology, Syracuse University, 130 College Place, Syracuse, NY 13244, USA.
Gene. 1998 Sep 18;218(1-2):103-9. doi: 10.1016/s0378-1119(98)00359-x.
We have cloned and sequenced the green opsin genes of rabbit and rat. When these genes are expressed in cultured cells and reconstituted with 11-cis retinal, the resulting visual pigments have wavelengths of maximal absorption (lambdamax) of 509nm. These blue-shifted lambdamax values are fully explained by their aa composition A, Y, Y, T, and S at sites 180, 197, 277, 285, and 308, respectively. The inference on the ancestral pigment sequences strongly suggests that the rabbit and rat (and mouse) green pigments attained the extant lambdamax values independently, mainly by the single aa replacement A308S.
我们已经克隆并测序了兔子和大鼠的绿色视蛋白基因。当这些基因在培养细胞中表达并与11-顺式视黄醛重组时，产生的视觉色素的最大吸收波长（λmax）为509nm。这些蓝移的λmax值可以完全由它们在第180、197、277、285和308位的氨基酸组成A、Y、Y、T和S来解释。对祖先色素序列的推断强烈表明，兔子和大鼠（以及小鼠）的绿色色素主要通过单个氨基酸替换A308S独立获得了现有的λmax值。
