Dziadziuszko H, Kunikowska D, Głośnicka R, Gajdus J, Kaczyński Z, Szafranek J
Institute of Maritime and Tropical Medicine, Gdynia, Poland.
FEMS Immunol Med Microbiol. 1998 Aug;21(4):253-9. doi: 10.1111/j.1574-695X.1998.tb01172.x.
Lipopolysaccharide of Salmonella haarlem was hydrolyzed and the products separated. Native O-polysaccharide antigen was oxidised with sodium periodate followed by reduction with sodium borohydride. Native and chemically modified antigens were the subject of immunochemical studies. Monoclonal antibodies against S. haarlem and polyclonal rabbit antisera against S. haarlem, S. typhi and S. anatum bacteria were produced. The serological relationship between the lipopolysaccharide of Salmonella bacteria belonging to the two different groups D2 and E1 was demonstrated using haemagglutination reactions, inhibition of haemagglutination and immunoblotting. Cross-reactions were observed in haemagglutination reactions and in immunoblotting between antisera to S. haarlem, S. typhi and S. anatum. Factors 3,9,46 were found in the S. haarlem strain and the sugar composition for the epitopes of each factor was determined.
哈勒姆沙门氏菌的脂多糖被水解，产物得以分离。天然O-多糖抗原先用高碘酸钠氧化，随后用硼氢化钠还原。天然和化学修饰的抗原成为免疫化学研究的对象。制备了针对哈勒姆沙门氏菌的单克隆抗体以及针对哈勒姆沙门氏菌、伤寒沙门氏菌和鸭沙门氏菌的兔多克隆抗血清。利用血凝反应、血凝抑制和免疫印迹法证明了属于两个不同组（D2和E1）的沙门氏菌细菌脂多糖之间的血清学关系。在针对哈勒姆沙门氏菌、伤寒沙门氏菌和鸭沙门氏菌的抗血清的血凝反应和免疫印迹中观察到了交叉反应。在哈勒姆沙门氏菌菌株中发现了3、9、46因子，并确定了每个因子表位的糖组成。
