Mitterer M, Mair W, Gatti D, Sheldon J, Vachula M, Coser P, Schultz T F
Department of Haematology and Bone Marrow Transplantation Centre, Bozen/Bolzano, Italy.
Br J Haematol. 1998 Sep;102(5):1338-40. doi: 10.1046/j.1365-2141.1998.00894.x.
The aim of our study was to test if dendritic cells contain the KSHV genome. CD34+ peripheral blood progenitor cells (PBPC) and bone marrow mononuclear cells were cultured in X-VIVO 15 medium supplemented with GM-CSF and TNF-alpha in gas-permeable containers. Dendritic cells were identified morphologically and immunophenotypically. The KSHV genome was not identified in any of the cases using a nested primer PCR approach. Serological analysis corroborated the molecular findings: no antibodies for KSHV were found in any of the multiple myeloma patients. These data are of importance when considering use of DC for therapeutic approaches in multiple myeloma.
