Fourier transform infrared study of the cation radical of P680 in the photosystem II reaction center: evidence for charge delocalization on the chlorophyll dimer.

A Fourier transform infrared (FTIR) difference spectrum of the primary electron donor (P680) of photosystem II upon its photooxidation (P680+/P680) was obtained in the frequency region of 1000-3000 cm-1. The reaction center (RC) complex (D1-D2-Cytb559) was used for the measurements in the presence of ferricyanide as an exogenous electron acceptor. Control measurements of electronic absorption (300-1200 nm) showed that illumination of the RC complex at 150 K induced major oxidation of P680 concomitant with oxidation of a carotenoid and an accessory chlorophyll (Chl). Illumination at 250 K also specifically bleached one of the two beta-carotene molecules bound to the RC complex, and the sample thus treated exhibited little formation of a carotenoid cation on subsequent illumination at 150 K. The P680+/P680 FTIR difference spectrum (with minor contamination of Chl+/Chl) was measured at 150 K using this partially carotenoid-deficient RC complex. The spectrum showed a broad positive band centered at approximately 1940 cm-1, which could be ascribed to an infrared electronic transition of P680+ analogous to that previously observed in various bacterial P+. This finding indicates that a positive charge is delocalized over (or hopping between) the two Chl molecules in P680+. The low intensity of this electronic band compared with that of the bacterial band could have three possible explanations: weak resonance interaction between the constituent Chl molecules, an asymmetric structure of P680+, and the difference in Chl species. Bands in the C=O stretching region (1600-1750 cm-1) were interpreted in comparison with resonance Raman spectra of the RC complex. The negative peaks at 1704 and 1679 cm-1 were proposed as candidates for the keto C9=O bands of P680. The observation that neither of these bands agreed with the main keto C9=O band at 1669 cm-1 in the previous 3P680/P680 FTIR spectrum [Noguchi et al. (1993) Biochemistry 32, 7186-7195] led to the idea that the triplet state migrates to a Chl (designated as ChlT) different from P680 at low temperatures. Based on these results, structural models of Chl molecules including P680 and ChlT and their coupling in the cation, triplet, and Qy singlet states are discussed.