Ikekubo K
Department of Nuclear Medicine, Kobe City General Hospital.
Kaku Igaku. 1998 Jul;35(6):357-66.
The history of development of in vitro radioassays is reviewed. The basic principles and clinical uses of saturation analysis, competitive radioassay and immunoradiometric assay are described. The more rapid, sensitive, specific and accurate assays are required at all times. However, it is well known that autoantibodies or heterophilic antibodies in serum interfere with radioassay and cause false results. Here I present the technical problems of the assay in the presence of such interfering substances which need to be resolved and also discussed the discrepancy between bioactivity and immunoreactivity in the assay.
本文回顾了体外放射分析的发展历程。描述了饱和分析、竞争性放射分析和免疫放射分析的基本原理及临床应用。一直以来都需要更快速、灵敏、特异且准确的分析方法。然而，众所周知，血清中的自身抗体或嗜异性抗体可干扰放射分析并导致错误结果。在此，我提出了在存在此类干扰物质时该分析方法所面临的需要解决的技术问题，并讨论了该分析中生物活性与免疫反应性之间的差异。
