el-Shabrawi Y, Livir-Rallatos C, Christen W, Baltatzis S, Foster C S
Uveitis and Immunology Service, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston 02114, USA.
Ophthalmology. 1998 Sep;105(9):1659-63. doi: 10.1016/S0161-6420(98)99035-2.
This study aimed to investigate the role of interleukin-12 (IL-12) and interleukin-10 (IL-10) in initiation and maintenance of intraocular inflammation.
Case series.
Aqueous humor and vitreous levels of IL-12 and IL-10 were measured in 22 patients with uveitis undergoing cataract surgery, paracentesis of the anterior chamber, and/or vitrectomy for diagnostic reasons, and in 4 patients with cataract only.
Aqueous humor and vitreous levels of IL-12 and IL-10 were measured with specific enzyme-linked immunosorbent assays.
Disease activity was correlated to IL-12 levels in the aqueous humor and the vitreous of patients with uveitis.
Cytokine levels found in the anterior chamber and the vitreous are presented in picogram/milliliter (medium; range). The highest IL-12 levels were found in patients with active uveitis (108.5 pg/ml; 72-293 pg/ml). Interleukin-12 in patients with moderate uveitis or with their disease in remission was lower (32 pg/ml; 15-94 pg/ml) than in patients with active disease (P > 0.001) but higher than in the control group (10.5 pg/ml; 9-14 pg/ml). Interleukin-10 was detectable in only 3 of 22 patients with uveitis (12 pg/ml; 9-23 pg/ml).
The authors found statistically significant differences of IL-12 levels in the various patient groups (active vs. inactive vs. control). These results support the idea that these uveitis cases represent type 1 (Th1)-T lymphocyte-mediated diseases in which IL-12 plays a pivotal role in the initiation and maintenance of the intraocular inflammation. The high levels of IL-12 in the vitreous and/or aqueous humor of the patients with uveitis suggest that susceptibility or resistance to ocular autoimmunity may be connected to a genetic predisposition to an elevated Th1 response.
本研究旨在探讨白细胞介素-12（IL-12）和白细胞介素-10（IL-10）在眼内炎症的起始和维持过程中的作用。
病例系列。
对22例因诊断需要接受白内障手术、前房穿刺和/或玻璃体切割术的葡萄膜炎患者以及4例仅患有白内障的患者，检测其房水和玻璃体内IL-12和IL-10的水平。
采用特异性酶联免疫吸附测定法检测房水和玻璃体内IL-12和IL-10的水平。
葡萄膜炎患者房水和玻璃体内的疾病活动度与IL-12水平的相关性。
前房和玻璃体内发现的细胞因子水平以皮克/毫升（中位数；范围）表示。活动性葡萄膜炎患者的IL-12水平最高（108.5 pg/ml；72 - 293 pg/ml）。中度葡萄膜炎患者或病情缓解患者的白细胞介素-12水平（32 pg/ml；15 - 94 pg/ml）低于活动性疾病患者（P > 0.001），但高于对照组（10.5 pg/ml；9 - 14 pg/ml）。22例葡萄膜炎患者中仅3例可检测到白细胞介素-10（12 pg/ml；9 - 23 pg/ml）。
作者发现不同患者组（活动性与非活动性与对照组）的IL-12水平存在统计学显著差异。这些结果支持了这些葡萄膜炎病例代表1型（Th1）-T淋巴细胞介导疾病的观点，其中IL-12在眼内炎症的起始和维持中起关键作用。葡萄膜炎患者玻璃体液和/或房水中IL-12水平较高表明，对眼部自身免疫的易感性或抵抗力可能与Th1反应升高的遗传易感性有关。
