Darbès J, Majzoub M, Breuer W, Hermanns W
Institute for Veterinary Pathology, University of Munich, Germany.
Vet Pathol. 1998 Sep;35(5):370-9. doi: 10.1177/030098589803500506.
This report describes six cases of feline large granular lymphocyte lymphoma identified by light microscopy on the basis of their characteristic azurophilic granulation in Giemsa-stained plastic sections and by electron microscopy on the basis of their typical granules. Although the granules of all the tumor cells were negative for peroxidase activity, they all demonstrated chloroacetate-esterase and acid phosphatase activity. All the tumors reacted with cross-reacting antibodies against the CD3 antigen (epsilon chain) and did not react with a cross-reacting monoclonal antibody directed against epitopes on cytoplasmic domains of the CD20 antigen. Three tumors had a positive reaction with a monoclonal human CD57-like antibody. This is highly suggestive of either a cytotoxic T cell or a natural killer cell origin of the neoplasias. In three cats, although other abdominal organs were affected to a variable extent, the main neoplastic lesions were localized in the gastrointestinal tract and the jejunal lymph nodes. In contrast, in the other three cats, organ involvement was more widespread, affecting the lung (two), myocardium (two), precardiac mediastinum (one), salivary gland (one), and spinal cord (one); in addition, leukemia was present in two of these cats. The data presented indicate that tumors made up of large granular lymphocytes occur more frequently in cats than previously assumed and that they share many characteristic features with specific subtypes of clonal disorders of large granular lymphocytes in humans.
本报告描述了6例猫大颗粒淋巴细胞淋巴瘤病例，这些病例通过光学显微镜在吉姆萨染色塑料切片中基于其特征性嗜天青颗粒而得以识别，同时通过电子显微镜基于其典型颗粒得以识别。尽管所有肿瘤细胞的颗粒过氧化物酶活性均为阴性，但它们均表现出氯乙酸酯酶和酸性磷酸酶活性。所有肿瘤均与抗CD3抗原（ε链）的交叉反应抗体发生反应，而不与针对CD20抗原胞质结构域表位的交叉反应单克隆抗体发生反应。3例肿瘤与单克隆人CD57样抗体呈阳性反应。这强烈提示这些肿瘤来源于细胞毒性T细胞或自然杀伤细胞。在3只猫中，尽管其他腹部器官受到不同程度的影响，但主要肿瘤病变位于胃肠道和空肠淋巴结。相比之下，在另外3只猫中，器官受累更为广泛，累及肺（2例）、心肌（2例）、心前纵隔（1例）、唾液腺（1例）和脊髓（1例）；此外，其中2只猫存在白血病。所呈现的数据表明，由大颗粒淋巴细胞组成的肿瘤在猫中比以前认为的更常见，并且它们与人类大颗粒淋巴细胞克隆性疾病的特定亚型具有许多共同特征。
