Thompson K D
Department of Pathology, The University of Chicago Medical Center, IL 60637, USA.
Antiviral Res. 1998 Jul;39(1):55-61. doi: 10.1016/s0166-3542(98)00027-8.
Viracea, a topical microbicide, is a blend of benzalkonium chloride and phytochemicals derived from Echinacea purpurea and is a proprietary formula from Destiny BioMediX Corp. Viracea was tested against 40 strains of herpes simplex virus (HSV): 15 strains (five HSV-1 and ten HSV-2) were resistant to acyclovir (ACV-R) and 25 strains (13 HSV-1 and 12 HSV-2) were susceptible to ACV (ACV-S). The median ED50 of Viracea for the five ACV-R strains of HSV-1 was a 1:100 dilution of the drug with a range of 1:50-1:400. The median ED50 of Viracea for the ten ACV-R strains of HSV-2 was 1:200 with a range of 1:50-1:3200. For the ACV-S strains of HSV-1 and HSV-2, the median ED50 of Viracea was 1:100 and 1:200, respectively. The cytotoxicity of Viracea was evaluated in a standard neutral red dye uptake assay in human foreskin fibroblasts. The cytotoxicity of Viracea approached only 50% at the highest concentration of the drug tested, a 1:2 dilution, indicating that Viracea is non-toxic in this cell cytotoxicity assay. Although the active component(s) in Viracea that has anti-HSV activity is not known, it appears that this extract has good antiviral activity against both ACV resistant and ACV susceptible strains of HSV-1 and HSV-2.
Viracea是一种局部用杀菌剂，是苯扎氯铵与紫锥菊中提取的植物化学物质的混合物，是Destiny BioMediX公司的专利配方。Viracea针对40株单纯疱疹病毒（HSV）进行了测试：15株（5株HSV - 1和10株HSV - 2）对阿昔洛韦耐药（ACV - R），25株（13株HSV - 1和12株HSV - 2）对阿昔洛韦敏感（ACV - S）。Viracea对5株ACV - R的HSV - 1毒株的半数有效剂量（ED50）中位数为药物1:100稀释液，范围为1:50 - 1:400。Viracea对10株ACV - R的HSV - 2毒株的ED50中位数为1:200，范围为1:50 - 1:3200。对于ACV - S的HSV - 1和HSV - 2毒株，Viracea的ED50中位数分别为1:100和1:200。在人包皮成纤维细胞中通过标准中性红染料摄取试验评估了Viracea的细胞毒性。在测试的最高药物浓度即1:2稀释液时，Viracea的细胞毒性仅接近50%，表明Viracea在该细胞毒性试验中无毒。尽管Viracea中具有抗HSV活性的活性成分尚不清楚，但该提取物似乎对HSV - 1和HSV - 2的阿昔洛韦耐药株和阿昔洛韦敏感株均具有良好的抗病毒活性。
