Matorras R, Ruiz J I, Mendoza R, Ruiz N, Sanjurjo P, Rodriguez-Escudero F J
Department of Obstetrics and Gynecology, Hospital from Cruces, Pais Vasco University, Baracaldo, Vizcaya, Spain.
Hum Reprod. 1998 Aug;13(8):2227-30. doi: 10.1093/humrep/13.8.2227.
The aim of the study was to assess the fatty acid composition of human fertilization-failed oocytes. A total of 150 unfertilized oocytes from 43 women undergoing in-vitro fertilization (IVF) were analysed using capillary gas chromatography. The majority of fatty acids were saturated (79.22%), of which stearic (38.65%) and palmitic (32.66%) acids were the most abundant. Of the monounsaturated fatty acids (14.27%) oleic acid was the most abundant (9.77%). Polyunsaturated fatty acids comprised 6.50% of fatty acids, the n-6:n-3 ratio being 7.73. The ratio of eicosapentaenoic acid:docosahexaenoic acid was approximately 5. It is concluded that the most common fatty acids in human unfertilized oocytes are either saturated or monounsaturated fatty acids, whose main function is to provide an energy source. A number of differences in fatty acid composition were observed, in comparison with other biological samples. In particular, stearic and eicosapentaenoic acids were more prominent, and oleic and linoleic acids were less prominent; this may reflect some specific peculiarity of oocyte metabolism.
本研究的目的是评估人类受精失败的卵母细胞的脂肪酸组成。使用毛细管气相色谱法对43名接受体外受精（IVF）的女性的总共150个未受精的卵母细胞进行了分析。大多数脂肪酸为饱和脂肪酸（79.22%），其中硬脂酸（38.65%）和棕榈酸（32.66%）含量最高。在单不饱和脂肪酸（14.27%）中，油酸含量最高（9.77%）。多不饱和脂肪酸占脂肪酸的6.50%，n-6:n-3比例为7.73。二十碳五烯酸与二十二碳六烯酸的比例约为5。结论是，人类未受精卵母细胞中最常见的脂肪酸是饱和脂肪酸或单不饱和脂肪酸，其主要功能是提供能量来源。与其他生物样本相比，观察到脂肪酸组成存在一些差异。特别是，硬脂酸和二十碳五烯酸更为突出，油酸和亚油酸则不那么突出；这可能反映了卵母细胞代谢的一些特定特性。
