Brown G M, Huckerby T N, Bayliss M T, Nieduszynski I A
Department of Biological Sciences, Institute of Environmental and Natural Sciences, Lancaster University, Bailrigg, Lancaster, LA1 4YQ United Kingdom.
J Biol Chem. 1998 Oct 9;273(41):26408-14. doi: 10.1074/jbc.273.41.26408.
Alkaline borohydride-reduced keratan sulfate chains were isolated from human articular cartilage aggrecan from individuals of various ages (0-85 years old). The chains were structurally characterized using 1H NMR spectroscopy, gel permeation chromatography, and oligosaccharide profiling (after digestion with the enzymes keratanase and keratanase II). The results show that from birth to early adolescence (0-9 years) the levels of alpha(1-3)-fucosylation, alpha(2-3)-sialylation, and galactose sulfation increase. Also, the weight-average molecular weight of the chains increases. During maturation (9-18 years) the levels of fucosylation and galactose sulfation continue to increase and alpha(2-6)-sialylation of the chains occurs. In adult life (18-85 years) there is little change in the weight-average molecular weight of the chains, and the levels of fucosylation, sialylation, and sulfation remain fairly constant.
从不同年龄（0 - 85岁）个体的人关节软骨聚集蛋白聚糖中分离出碱性硼氢化物还原的硫酸角质素链。使用核磁共振氢谱（1H NMR）光谱、凝胶渗透色谱法和寡糖谱分析（用角质素酶和角质素酶II消化后）对这些链进行结构表征。结果表明，从出生到青春期早期（0 - 9岁），α(1 - 3)-岩藻糖基化、α(2 - 3)-唾液酸化和半乳糖硫酸化水平增加。此外，链的重均分子量增加。在成熟阶段（9 - 18岁），岩藻糖基化和半乳糖硫酸化水平继续增加，并且链发生α(2 - 6)-唾液酸化。在成年期（18 - 85岁），链的重均分子量几乎没有变化，岩藻糖基化、唾液酸化和硫酸化水平保持相当恒定。
