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利用洋葱根尖后期-末期染色体畸变试验对污水污泥进行遗传毒性测试。

Genotoxicity testing of wastewater sludge using the Allium cepa anaphase-telophase chromosome aberration assay.

作者信息

Rank J, Nielsen M H

机构信息

Department of Environment, Technology and Social Studies, Roskilde University, PO Box 260, DK-4000, Roskilde, Denmark.

出版信息

Mutat Res. 1998 Oct 12;418(2-3):113-9. doi: 10.1016/s1383-5718(98)00118-1.

DOI:10.1016/s1383-5718(98)00118-1
PMID:9757013
Abstract

Wastewater sludges were analysed in the Allium cepa genotoxicity test. They were sampled during three winter periods from three Danish municipal wastewater treatment plants differing in size and industrial load. The toxicity of the sludge was tested in the Allium root inhibition assay, and the results expressed as EC30 and EC50 values showed that the toxicity could be positive correlated to the industrial load. However, when genotoxicity was tested at concentrations corresponding to the EC30 and EC50 values in the A. cepa anaphase-telophase assay, only two sludge samples from the smallest plant with the lowest industrial load induced significant chromosome aberrations. Concentrations of the heavy metal's Pb, Ni, Cr, Zn, Cu, and Cd were also determined and could partly be correlated with the toxicity of the sludge and the industrial load of the treatment plants.

摘要

采用洋葱(Allium cepa)遗传毒性试验对废水污泥进行了分析。在三个冬季期间,从丹麦三座规模和工业负荷不同的城市污水处理厂采集了污泥样本。通过洋葱根抑制试验对污泥的毒性进行了测试,以EC30和EC50值表示的结果表明,毒性与工业负荷呈正相关。然而,当在洋葱后期 - 末期试验中以对应于EC30和EC50值的浓度测试遗传毒性时,只有来自工业负荷最低的最小污水处理厂的两个污泥样本诱导了显著的染色体畸变。还测定了重金属铅(Pb)、镍(Ni)、铬(Cr)、锌(Zn)、铜(Cu)和镉(Cd)的浓度,其浓度部分与污泥的毒性和污水处理厂的工业负荷相关。

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