Veenstra T D, Prüfer K, Koenigsberger C, Brimijoin S W, Grande J P, Kumar R
Nephrology Research Unit, Mayo Clinic/Foundation, Rochester, MN 55905, USA.
Brain Res. 1998 Sep 7;804(2):193-205. doi: 10.1016/s0006-8993(98)00565-4.
We have mapped areas within the central nervous system (CNS) of the developing fetal rat which immunostain for the 1,25-dihydroxyvitamin D3 receptor (VDR). The VDR was detected from days 12 to 21 of gestation throughout the CNS; immunostaining was particularly intense in the neuroepithelium and within the differentiating fields of various areas of the brain. Cells within the spinal cord, dorsal root, and other ganglia exhibited positive staining for the VDR. The intensity of staining for the VDR diminished or disappeared in the neuroepithelium throughout the CNS during the later days of development, while in the differentiating fields single VDR immunoreactive cells were observed. The presence of the VDR in the CNS was confirmed by in situ hybridization and RNA-based polymerase chain reaction methods with di-deoxy sequencing of the resultant DNA product. These results support the hypothesis that 1, 25-dihydroxyvitamin D3, through interactions with the VDR, may play a role in the development of the CNS.
我们已绘制出发育中胎鼠中枢神经系统（CNS）内对1,25 - 二羟维生素D3受体（VDR）进行免疫染色的区域。在整个妊娠期的第12天至21天，整个中枢神经系统均检测到VDR；免疫染色在神经上皮以及大脑各个区域的分化区域中尤为强烈。脊髓、背根和其他神经节内的细胞对VDR呈阳性染色。在发育后期，整个中枢神经系统神经上皮中VDR的染色强度减弱或消失，而在分化区域中观察到单个VDR免疫反应性细胞。通过原位杂交和基于RNA的聚合酶链反应方法以及对所得DNA产物进行双脱氧测序，证实了中枢神经系统中VDR的存在。这些结果支持以下假设，即1,25 - 二羟维生素D3通过与VDR相互作用，可能在中枢神经系统的发育中发挥作用。
