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使用新型动态光散射检测器检测溶菌酶成核作用。

Accessing lysozyme nucleation with a novel dynamic light scattering detector.

作者信息

Peters R, Georgalis Y, Saenger W

机构信息

ALV-Laser Vertiebsgesellschaft mbH, Robert-Bosch-Strasse 46, D-63225 Langen, Germany.

出版信息

Acta Crystallogr D Biol Crystallogr. 1998 Sep 1;54(Pt 5):873-7. doi: 10.1107/s0907444998002455.

DOI:10.1107/s0907444998002455
PMID:9757103
Abstract

Dynamic light scattering was employed to investigate the behaviour of nucleating hen egg-white lysozyme solutions. For these studies we employed a novel fiber-optic based microprobe that suppresses multiple light scattering and contributions from large clusters in the spectra by backscattering detection. The time evolution of small lysozyme clusters is found to obey classical nucleation at the initial stages of the reaction.

摘要

采用动态光散射技术研究了成核的鸡蛋清溶菌酶溶液的行为。在这些研究中，我们使用了一种新型的基于光纤的微探针，该微探针通过背散射检测抑制了光谱中的多重光散射和大聚集体的贡献。发现在反应初始阶段，小溶菌酶聚集体的时间演化遵循经典成核过程。

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