Osakabe N, Sanbongi C, Yamagishi M, Takizawa T, Osawa T
Functional Food Research and Development Laboratories, Meiji Seika Kaisha, Ltd., Saitama, Japan.
Biosci Biotechnol Biochem. 1998 Aug;62(8):1535-8. doi: 10.1271/bbb.62.1535.
The antiulcer activity of cacao liquor water-soluble crude polyphenols (CWSP) was examined. CWSP, alpha-tocopherol, sucralfate (500 mg/kg), and cimetidine (250 mg/kg) were orally administered to male SD rats 30 minutes before ethanol treatment. 5 ml/kg of ethanol given intragastrically caused lesions in mucosa of the glandular stomach. CWSP caused a reduction of such hemorrhagic lesions as well as cimetidine and sucralfate which are typical antiulcer drugs, but alpha-tocopherol was less effective. Thiobarbituric acid reactive substances in gastric mucosa significantly increased with ethanol administration. CWSP treatment significantly reduced this change. The administration of ethanol extensively increased myeloperoxidase (MPO) but not xanthine oxidase (XOD) activity. CWSP reduced the activities of both enzymes; they were considered the main sources of oxygen radicals. According to an in vitro study, CWSP directly reducted XOD but not MPO. These results suggest that the antiulcer mechanism of CWSP was not only radical scavenging but also modulation of leukocyte function.
对可可液块水溶性粗多酚（CWSP）的抗溃疡活性进行了研究。在乙醇处理前30分钟，将CWSP、α-生育酚、硫糖铝（500毫克/千克）和西咪替丁（250毫克/千克）口服给予雄性SD大鼠。胃内给予5毫升/千克乙醇会导致腺胃黏膜损伤。CWSP与典型抗溃疡药物西咪替丁和硫糖铝一样，可减少此类出血性损伤，但α-生育酚的效果较差。乙醇给药后，胃黏膜中的硫代巴比妥酸反应性物质显著增加。CWSP处理可显著减少这种变化。乙醇给药会大幅增加髓过氧化物酶（MPO）活性，但不会增加黄嘌呤氧化酶（XOD）活性。CWSP可降低这两种酶的活性；它们被认为是氧自由基的主要来源。根据体外研究，CWSP可直接还原XOD，但不能还原MPO。这些结果表明，CWSP的抗溃疡机制不仅包括清除自由基，还包括调节白细胞功能。
