Gajkowska B, Walski M, Olszewska H
Laboratory of the Ultrastructure of the Nervous System, Medical Research Centre, Polish Academy of Sciences, Warszawa.
Folia Neuropathol. 1998;36(2):109-16.
Here, using a histochemical staining for a microglia/phagocyte marker TPP-ase (Murabe, Sano 1981), and an electron microscopy we characterized the population of pituitary phagocytic cells activated by cerebral ischemia. An intense thiamine pyrophosphatase (TPP-ase) activity was demonstrated in glial cells and some cells of blood vessels of neural lobe, late period (12 months) after experimental ischemia. TPP-ase positive cells were ultrastructurally identified as pituicytes, microglia, pericytes and perivascular cells. The product characteristic for TPP-ase activity was seen on plasma membrane of these cells. Our electron-microscopic histochemical results provide strong support for a role of pituicytes, pericytes and perivascular cells as a phagocytic cells involved in mechanism of elimination of ischemically damaged axonal endings in neural lobe.
在此，我们利用一种针对小胶质细胞/吞噬细胞标志物硫胺素焦磷酸酶（TPP - 酶）的组织化学染色方法（村部、佐野，1981年）以及电子显微镜技术，对脑缺血激活的垂体吞噬细胞群体进行了特征描述。在实验性缺血后的晚期（12个月），神经叶的神经胶质细胞和一些血管细胞中显示出强烈的硫胺素焦磷酸酶（TPP - 酶）活性。TPP - 酶阳性细胞在超微结构上被鉴定为垂体细胞、小胶质细胞、周细胞和血管周围细胞。在这些细胞的质膜上可见TPP - 酶活性的产物特征。我们的电子显微镜组织化学结果有力地支持了垂体细胞、周细胞和血管周围细胞作为吞噬细胞参与神经叶缺血性损伤轴突终末清除机制的作用。
