Walski M, Gajkowska B
The Laboratory of the Ultrastructure of the Nervous System, Medical Research Centre, Polish Academy of Sciences, Warsaw.
J Hirnforsch. 1998;39(2):183-91.
Brain phagocytes are members of a heterogeneous family of microglial cells. In this study we investigated the membrane activity of the enzyme, thiamine pyrophosphatase (TPP-ase), in brain phagocytes. Studies were performed on rats subjected to a transient ischemia because ischemic incident precipitates proliferation of microglial cells in the brain. Brain tissue was sampled from animals that survived 12 months after experimentally evoked cardiac arrest. The product characteristic for TPP-ase activity was present in the Golgi cisterns of neurons, basement membranes of endothelia and capillary pericytes. TPP-ase activity was present on plasma membranes of brain phagocytes. The phagocyte TPP-ase activity did not depend on the anatomical localization of the cell in the brain (cortex, hippocampus, hypothalamus). Thus, TPP-ase activity can be considered as a marker of brain phagocytes.
脑吞噬细胞是小胶质细胞异质性家族的成员。在本研究中，我们研究了硫胺素焦磷酸酶（TPP酶）在脑吞噬细胞中的膜活性。对经历短暂缺血的大鼠进行了研究，因为缺血事件会促使脑内小胶质细胞增殖。脑组织取自实验性诱发心脏骤停后存活12个月的动物。TPP酶活性的产物特征存在于神经元的高尔基池中、内皮细胞的基底膜和毛细血管周细胞中。TPP酶活性存在于脑吞噬细胞的质膜上。吞噬细胞的TPP酶活性不依赖于细胞在脑内的解剖定位（皮质、海马体、下丘脑）。因此，TPP酶活性可被视为脑吞噬细胞的标志物。
