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嗜盐栖热单胞菌中由外源胆碱合成甘氨酸甜菜碱

Synthesis of glycine betaine from exogenous choline in the moderately halophilic bacterium halomonas elongata.

作者信息

Canovas D, Vargas C, Csonka LN, Ventosa A, Nieto JJ

机构信息

Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, 41012 Seville, Spain.

出版信息

Appl Environ Microbiol. 1998 Oct;64(10):4095-7. doi: 10.1128/AEM.64.10.4095-4097.1998.

Abstract

The role of choline in osmoprotection in the moderate halophile Halomonas elongata has been examined. Transport and conversion of choline to betaine began immediately after addition of choline to the growth medium. Intracellular accumulation of betaine synthesized from choline was salt dependent up to 2.5 M NaCl. Oxidation of choline was enhanced at 2.0 M NaCl in the presence or absence of externally provided betaine. This indicates that the NaCl concentration in the growth medium has major effects on the choline-betaine pathway of H. elongata.

摘要

已对胆碱在中度嗜盐菌嗜盐栖热袍菌渗透保护中的作用进行了研究。向生长培养基中添加胆碱后,胆碱向甜菜碱的转运和转化立即开始。由胆碱合成的甜菜碱在细胞内的积累在高达2.5 M NaCl的盐浓度下是依赖于盐的。在存在或不存在外部提供的甜菜碱的情况下,2.0 M NaCl时胆碱的氧化增强。这表明生长培养基中的NaCl浓度对嗜盐栖热袍菌的胆碱-甜菜碱途径有重大影响。

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