Rakich D R, Wataha J C, Lefebvre C A, Weller R N
Department of Oral Rehabilitation, School of Dentistry, Medical College of Georgia, Augusta 30912-1260, USA.
J Endod. 1998 Aug;24(8):528-33. doi: 10.1016/S0099-2399(98)80071-X.
Dentin bonding agents (DBA) have been considered for use as root-end fillings. Previous studies have documented the release of DBA components in vivo and in vitro, but the biological implications are not clear. The macrophage is important in wound healing, and likely to be important in any inflammatory response. Therefore, this study determined the concentrations of the components of DBAs that suppress the mitochondrial activity of human macrophages in vitro. THP-1 macrophages were cultured in the presence of four DBA components (2-hydroxyethyl methacrylate (HEMA), 4-methacryloxyethyl trimellitate anhydride (4-META), bisphenol-glycidylmethacrylate (Bis-GMA), and urethane dimethacrylate (UDMA)) at various concentrations and for varying durations. Residual effects were also measured after the resins were removed. Controls received only the vehicle solution, ethanol or water. THP-1 mitochondrial activity was estimated using the MTT assay, and the 50% toxicity concentrations (TC50) were determined graphically. Resin components suppressed the mitochondrial activity of macrophages at different concentrations (TC50 values for HEMA (10,000 mumol/L), 4-META (3,800 mumol/L), Bis-GMA (130 mumol/L), and UDMA (110 mumol/L) at 24 h, and the effect was time-dependent. Residual effects were observed for all resins.
牙本质粘结剂（DBA）已被考虑用作根尖充填材料。以往的研究记录了DBA成分在体内和体外的释放情况，但其生物学意义尚不清楚。巨噬细胞在伤口愈合中很重要，并且可能在任何炎症反应中都很重要。因此，本研究确定了在体外抑制人巨噬细胞线粒体活性的DBA成分的浓度。将THP-1巨噬细胞在四种DBA成分（甲基丙烯酸2-羟乙酯（HEMA）、偏苯三酸三缩水甘油酯甲基丙烯酸酯（4-META）、双酚甲基丙烯酸缩水甘油酯（Bis-GMA）和二甲基丙烯酸聚氨酯（UDMA））存在下，于不同浓度和不同时间进行培养。在去除树脂后还测量了残留效应。对照组仅接受溶媒溶液、乙醇或水。使用MTT法评估THP-1线粒体活性，并通过图表确定50%毒性浓度（TC50）。树脂成分在不同浓度下抑制巨噬细胞的线粒体活性（24小时时HEMA的TC50值为10,000 μmol/L、4-META为3,800 μmol/L、Bis-GMA为130 μmol/L、UDMA为110 μmol/L），且该效应具有时间依赖性。所有树脂均观察到残留效应。
