da Silva João M F, Rodrigues José R, Camargo Carlos H R, Fernandes Virgilio Vilas Boas, Hiller Karl-Anton, Schweikl Helmut, Schmalz Gottfried
Department of Operative Dentistry, São Paulo State University, Avenida Engenheiro Francisco José Longo 777, Jd. São Dimas, São José dos Campos, São Paulo, Brazil,
Clin Oral Investig. 2014;18(2):607-13. doi: 10.1007/s00784-013-1000-9. Epub 2013 May 28.
Besides possessing good mechanical properties, dental materials should present a good biological behavior and should not injure the involved tissues. Bond strength and biocompatibility are both highly significant properties of dentin adhesives. For that matter, these properties of four generations of adhesive systems (Multi-Purpose/Single Bond/SE Plus/Easy Bond) were evaluated.
Eighty bovine teeth had their dentin exposed (500- and 200-μm thickness). Adhesive was applied on the dentin layer of each specimen. Following that, the microshearing test was performed for all samples. A dentin barrier test was used for the cytotoxicity evaluation. Cell cultures (SV3NeoB) were collected from testing materials by means of 200- or 500-μm-thick dentin slices and placed in a cell culture perfusion chamber. Cell viability was measured 24 h post-exposition by means of a photometrical test (MTT test).
The best bonding performance was shown by the single-step adhesive Easy Bond (21 MPa, 200 μm; 27 MPa, 500 μm) followed by Single Bond (15.6 MPa, 200 μm; 23.4 MPa, 500 μm), SE Plus (18.2 MPa, 200 μm; 20 MPa, 500 μm), and Multi-Purpose (15.2 MPa, 200 μm; 17.9 MPa, 500 μm). Regarding the cytotoxicity, Multi-Purpose slightly reduced the cell viability to 92% (200 μm)/93% (500 μm). Single Bond was reasonably cytotoxic, reducing cell viability to 71% (200 μm)/64% (500 μm). The self-etching adhesive Scotchbond SE decreased cell viability to 85% (200 μm)/71% (500 μm). Conversely, Easy Bond did not reduce cell viability in this test, regardless of the dentin thickness.
Results showed that the one-step system had the best bond strength performance and was the least toxic to pulp cells. In multiple-step systems, a correct bonding technique must be done, and a pulp capping strategy is necessary for achieving good performance in both properties.
The study showed a promising system (one-step self-etching), referring to it as a good alternative for specific cases, mainly due to its technical simplicity and good biological responses.
牙科材料除了具有良好的机械性能外，还应具有良好的生物学性能，且不应损伤相关组织。粘结强度和生物相容性都是牙本质粘结剂的重要性能。因此，对四代粘结系统（多功能粘结剂/单组分粘结剂/SE Plus粘结剂/易粘结剂）的这些性能进行了评估。
80颗牛牙暴露牙本质（厚度分别为500μm和200μm）。将粘结剂涂覆在每个样本的牙本质层上。随后，对所有样本进行微剪切试验。采用牙本质屏障试验进行细胞毒性评估。通过200μm或500μm厚的牙本质切片从测试材料中收集细胞培养物（SV3NeoB），并置于细胞培养灌注室中。暴露24小时后，通过光度测试（MTT试验）测量细胞活力。
一步法粘结剂易粘结剂表现出最佳的粘结性能（200μm厚时为21MPa，500μm厚时为27MPa），其次是单组分粘结剂（200μm厚时为15.6MPa，500μm厚时为23.4MPa）、SE Plus粘结剂（200μm厚时为18.2MPa，500μm厚时为20MPa）和多功能粘结剂（200μm厚时为15.2MPa，500μm厚时为17.9MPa）。关于细胞毒性，多功能粘结剂使细胞活力略有降低，降至92%（200μm）/93%（500μm）。单组分粘结剂具有一定的细胞毒性，使细胞活力降至71%（200μm）/64%（500μm）。自酸蚀粘结剂Scotchbond SE使细胞活力降至85%（200μm）/71%（500μm）。相反，在该试验中，无论牙本质厚度如何，易粘结剂均未降低细胞活力。
结果表明，一步法系统具有最佳的粘结强度性能，对牙髓细胞的毒性最小。在多步法系统中，必须采用正确的粘结技术，并且需要进行牙髓盖髓策略以在这两种性能上都取得良好表现。
该研究显示了一种有前景的系统（一步法自酸蚀粘结剂），称其为特定病例的良好替代方案，主要是由于其技术简单且生物学反应良好。
