Brown Thomas J, Duewer D L, Kline M C, Sharpless K E
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Clin Chim Acta. 1998 Aug 10;276(1):75-87. doi: 10.1016/s0009-8981(98)00103-x.
The concentrations of retinol, alpha-tocopherol, and trans-beta-carotene in lyophilized serum stored at -25 degrees C and -80 degrees C have been monitored for 10 years. There was no evidence of degradation of any of these compounds over the 10-year period. Retinol, alpha-tocopherol, and trans-beta-carotene were less stable at -25 degrees C in liquid-frozen serum than they were in lyophilized serum. At -80 degrees C, trans-beta-carotene levels were stable for up to 3 years of storage in liquid-frozen serum. Both retinol and alpha-tocopherol appeared stable in liquid-frozen serum for at least 5 years at -80 degrees C. The effect of repeated freeze/thaw cycles on retinol, alpha-tocopherol, trans-lycopene, and trans-beta-carotene in liquid-frozen and reconstituted lyophilized serum both stored at -20 degrees C was also studied. Retinol, alpha-tocopherol, trans-lycopene, and trans-beta-carotene in reconstituted lyophilized serum stored at -20 degrees C were stable for at least 3 days with minimal (< 5) freeze/thaw cycles.
在-25℃和-80℃储存的冻干血清中，视黄醇、α-生育酚和反式β-胡萝卜素的浓度已监测了10年。在这10年期间，没有证据表明这些化合物中的任何一种发生了降解。视黄醇、α-生育酚和反式β-胡萝卜素在-25℃的液态冷冻血清中比在冻干血清中稳定性更低。在-80℃，反式β-胡萝卜素水平在液态冷冻血清中储存长达3年都是稳定的。视黄醇和α-生育酚在-80℃的液态冷冻血清中至少5年都表现出稳定性。还研究了反复冻融循环对同样储存在-20℃的液态冷冻血清和复溶冻干血清中的视黄醇、α-生育酚、反式番茄红素和反式β-胡萝卜素的影响。储存在-20℃的复溶冻干血清中的视黄醇、α-生育酚、反式番茄红素和反式β-胡萝卜素在最少（<5次）冻融循环下至少3天是稳定的。
