Abiaka Clifford, Olusi Samuel, Simbeye Amos
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Kuwait University, Sulaibikhat.
Asia Pac J Clin Nutr. 2002;11(1):22-7. doi: 10.1046/j.1440-6047.2002.00260.x.
Serum concentrations of retinol, alpha-tocopherol, beta-carotene and lycopene were measured by reversed-phase high-performance liquid chromatography (r-P HPLC) in 260 randomly selected healthy adult Kuwaitis (159 men and 101 women) aged 18-63 years (mean 33.3 years) to established reference ranges of the micronutrient antioxidants. Total cholesterol concentrations were assayed by an enzymatic method to determine alpha-tocopherol: cholesterol ratios. The mean +/- SEM (micromol/L) for retinol, alpha-tocopherol, beta-carotene and lycopene were 1.76+/-0.02, 20.0+/-0.5, 0.52+/-0.03, 0.95+/-0.05, respectively. Compared to other populations, these data showed, on the whole, ordinary concentrations of beta-carotene, comparatively low concentrations of retinol and alpha-tocopherol and high concentrations of lycopene. Retinol concentrations were similar for both sexes, whereas alpha-tocopherol concentration was significantly (P < 0.0001) lower and the carotenoid levels (beta-carotene and lycopene) significantly higher (P < 0.0001) in women. Of the micronutrient antioxidants, alpha-tocopherol was most correlated with cholesterol (r = 0.492, P < 0.0001). beta-Carotene and lycopene were highly correlated with each other (r =0.744, P< 0.0001). Age was positively associated with beta-carotene (r = 0.214, P = 0.001) and lycopene (r = 239, P< 0.0001). Our data enabled us to establish a gender non-specific reference range for retinol and gender-specific reference ranges for alpha-tocopherol, beta-carotene and lycopene.
采用反相高效液相色谱法（r-P HPLC）测定了260名年龄在18至63岁（平均33.3岁）的随机选取的科威特健康成年人（159名男性和101名女性）血清中视黄醇、α-生育酚、β-胡萝卜素和番茄红素的浓度，以确定这些微量营养素抗氧化剂的参考范围。采用酶法测定总胆固醇浓度，以确定α-生育酚与胆固醇的比值。视黄醇、α-生育酚、β-胡萝卜素和番茄红素的平均±标准误（微摩尔/升）分别为1.76±0.02、20.0±0.5、0.52±0.03、0.95±0.05。与其他人群相比，这些数据总体显示β-胡萝卜素浓度一般，视黄醇和α-生育酚浓度相对较低，而番茄红素浓度较高。视黄醇浓度在两性中相似，而女性的α-生育酚浓度显著较低（P < 0.0001），类胡萝卜素水平（β-胡萝卜素和番茄红素）显著较高（P < o.0001）。在微量营养素抗氧化剂中，α-生育酚与胆固醇的相关性最高（r = 0.492，P < 0.0001）。β-胡萝卜素和番茄红素彼此高度相关（r = 0.744，P < 0.0001）。年龄与β-胡萝卜素（r = 0.214，P = 0.001）和番茄红素（r = 0.239，P < 0.0001）呈正相关。我们的数据使我们能够建立视黄醇的非性别特异性参考范围以及α-生育酚、β-胡萝卜素和番茄红素的性别特异性参考范围。
