Caltagarone J, Rhodes J, Honer W G, Bowser R
Department of Pathology, University of Pittsburgh School of Medicine, PA 15261, USA.
Neuroreport. 1998 Aug 24;9(12):2907-12. doi: 10.1097/00001756-199808240-00042.
Synaptic function is critical for cell-cell communication and the characterization of proteins that function during vesicle formation, transport and fusion events will yield further insight into the mechanisms of synaptic transmission. We have cloned and characterized a gene product expressed in human brain called hCDCrel-1. This protein is a new member of the septin family of gene products that functions during cytokinesis in lower eukaryotes. In this study we characterize the expression of the hCDCrel-1 gene and localize the hCDCrel-1 protein to neurons in adult human brain. hCDCrel-1 co-purifies with SNAP-25 and synaptophysin marked synaptosomes, suggesting a novel function for this gene family in the brain. Our data indicate that members of the septin family of proteins may function in synaptic vesicle transport, fusion or recycling events in the human brain.
突触功能对于细胞间通讯至关重要，对在囊泡形成、运输和融合过程中发挥作用的蛋白质进行表征，将有助于进一步深入了解突触传递的机制。我们已经克隆并表征了一种在人类大脑中表达的基因产物，称为hCDCrel-1。该蛋白是基因产物septin家族的新成员，在低等真核生物的胞质分裂过程中发挥作用。在本研究中，我们表征了hCDCrel-1基因的表达，并将hCDCrel-1蛋白定位到成人大脑中的神经元。hCDCrel-1与SNAP-25和标记突触小体的突触素共纯化，表明该基因家族在大脑中具有新功能。我们的数据表明，septin家族蛋白成员可能在人类大脑的突触小泡运输、融合或再循环事件中发挥作用。
