Anderson G, Jenkinson E J
Department of Anatomy, Medical School, University of Birmingham, Edgbaston, UK.
J Immunol Methods. 1998 Jul 1;216(1-2):155-63. doi: 10.1016/s0022-1759(98)00076-3.
The establishment of in vitro culture systems provides an accessible means to study events within the immune system. In contrast to either dispersed suspension or two-dimensional monolayer culture, the explantation of tissue fragments under organ culture conditions is, to date, the only method which allows essential three-dimensional cellular interactions to be maintained under conditions which permit controlled experimental manipulation in vitro. Recent modifications of explant technology, particularly within the area of fetal thymic organ culture, now allow the controlled reassociation of defined cellular subsets and manipulation of gene expression, under conditions where the functioning of both lymphoid and stromal cell types closely resembles that in vivo.
体外培养系统的建立为研究免疫系统内的事件提供了一种可及的手段。与分散悬浮培养或二维单层培养不同，迄今为止，在器官培养条件下对组织片段进行外植是唯一一种能够在允许体外进行可控实验操作的条件下维持基本三维细胞相互作用的方法。外植技术的最新改进，特别是在胎儿胸腺器官培养领域，现在能够在淋巴细胞和基质细胞类型的功能与体内情况非常相似的条件下，实现特定细胞亚群的可控重新组合以及基因表达的操控。
