Houmard J A, Weidner M L, Gavigan K E, Tyndall G L, Hickey M S, Alshami A
Human Performance Laboratory, Department of Exercise and Sport Science, East Carolina University, Greenville, North Carolina 27858, USA.
J Appl Physiol (1985). 1998 Oct;85(4):1337-41. doi: 10.1152/jappl.1998.85.4.1337.
The purpose of this study was to determine whether enzymatic and histochemical characteristics of human skeletal muscle are altered with aging. Tissues from the vastus lateralis (VL) and gastrocnemius were analyzed for citrate synthase (CS) activity and fiber type in 55 sedentary men (age range 18-80 yr). In this population, CS activity in the gastrocnemius was negatively related to age (r = -0. 32, P < 0.05); there was no relationship in the VL. Treadmill-determined maximal oxygen consumption was positively related (r = 0.40, P < 0.05) to CS in the gastrocnemius but not in the VL. CS activity in the gastrocnemius was 24% lower in the oldest (>/=60 yr, n = 10) vs. the youngest (</=30 yr; n = 12) men; there was no change in CS activity in the VL with aging. No changes in fiber type were evident with age in either muscle. These data suggest a reduction in oxidative enzyme activity in human skeletal muscle with the aging process; this relationship may be muscle-group specific.
本研究的目的是确定人类骨骼肌的酶学和组织化学特性是否会随着衰老而改变。对55名久坐不动的男性（年龄范围18 - 80岁）的股外侧肌（VL）和腓肠肌组织进行了柠檬酸合酶（CS）活性和纤维类型分析。在该人群中，腓肠肌的CS活性与年龄呈负相关（r = -0.32，P < 0.05）；股外侧肌中则无此关系。通过跑步机测定的最大摄氧量与腓肠肌中的CS呈正相关（r = 0.40，P < 0.05），但与股外侧肌中的CS无关。年龄最大的男性（≥60岁，n = 10）与最年轻的男性（≤30岁；n = 12）相比，腓肠肌中的CS活性低24%；股外侧肌中的CS活性不会随衰老而改变。两种肌肉的纤维类型均未随年龄出现明显变化。这些数据表明，随着衰老过程，人类骨骼肌中的氧化酶活性降低；这种关系可能具有肌肉群特异性。
