采用激光诱导荧光增强电荷耦合器件检测的儿茶酚胺毛细管电泳法。
Capillary electrophoresis of catecholamines with laser-induced fluorescence intensified charge-coupled device detection.
作者信息
Xiong S, Han H, Zhao R, Chen Y, Liu G
机构信息
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
出版信息
Biomed Chromatogr. 2001 Apr;15(2):83-8. doi: 10.1002/bmc.37.
A capillary electrophoresis-laser-induced fluorescence-intensified charge-coupled device system was used for the separation and determination of catecholamines. Optimization of derivatization and separation conditions was investigated in order to reach a high separation efficiency and sensitivity. All fluorecein isothiocyanate derivatives of catecholamines were satisfactorily separated within 12 min. The detection limits were in attomole ranges. This method allows determination of catecholamines with high separation efficiencies and sensitivity.
采用毛细管电泳-激光诱导荧光-增强型电荷耦合器件系统对儿茶酚胺进行分离和测定。为了达到高分离效率和灵敏度，对衍生化和分离条件进行了优化。儿茶酚胺的所有异硫氰酸荧光素衍生物在12分钟内得到了满意的分离。检测限在阿托摩尔范围内。该方法能够以高分离效率和灵敏度测定儿茶酚胺。
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