牛心泛醇 - 细胞色素c还原酶晶体在276K下X射线衍射分辨率高达2.8埃。

Crystals of bovine heart ubiquinol-cytochrome c reductase diffracting X-rays up to 2.8 A resolution at 276 K.

作者信息

Murakami S, Shinzawa-Itoh K, Yoshikawa S

机构信息

Institute for Protein Research, Osaka University, Yamada-Oka Suita, Osaka, Japan.

出版信息

Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):146-7. doi: 10.1107/s0907444997007245.

DOI:10.1107/s0907444997007245

PMID:9761840

Abstract

Bovine heart ubiquinol-cytochrome c reductase stabilized with sucrose monocaplate was crystallized with polyethylene glycol as the precipitant at 277 K. X-rays diffracted by the crystal were detected up to 2.8 A resolution at 266 K, without using a synchrotron source. The space group and cell dimensions are P61 or P65 and a = b = 128.5 and c = 715.7 A, respectively.

摘要

用蔗糖单硬脂酸酯稳定的牛心泛醇 - 细胞色素c还原酶在277 K下以聚乙二醇作为沉淀剂进行结晶。在266 K下，无需使用同步辐射源，晶体衍射的X射线可检测到2.8 Å的分辨率。空间群和晶胞尺寸分别为P61或P65，a = b = 128.5 Å，c = 715.7 Å。