Teplyakov A, Oliva G, Polikarpov I
EMBL, Hamburg Outstation, DESY, Notkestrasse 85, 22603 Hamburg, Germany.
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):610-4. doi: 10.1107/s0907444997020295.
Potential benefits of using short X-ray wavelengths for protein crystal data collection arise from a reduction in absorption errors and a decrease in radiation damage of a sample. On the other hand, at longer wavelengths one may benefit from an increase in scattering efficiency of a crystal and an increase in intensity of an incident beam at a given synchrotron beamline. For small and frozen crystals the negative effects of absorption and radiation damage would be minimized which may shift the balance of interests towards the use of longer wavelengths. Experiments carried out at EMBL beamlines at DESY (Hamburg) show an advantage of using wavelengths longer than 1 A for data collection from crystals of up to 0.5 mm.
使用短X射线波长进行蛋白质晶体数据收集的潜在益处源于吸收误差的减少和样品辐射损伤的降低。另一方面，在较长波长下，晶体的散射效率增加以及在给定同步加速器光束线上入射光束强度增加，可能会带来好处。对于小晶体和冷冻晶体，吸收和辐射损伤的负面影响将降至最低，这可能会使人们更倾向于使用较长波长。在德国电子同步加速器研究所（汉堡）的欧洲分子生物学实验室光束线上进行的实验表明，对于尺寸达0.5毫米的晶体，使用波长大于1埃的数据收集具有优势。
