Vadlamudi R K, Shin J
Division of Tumor Virology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
FEBS Lett. 1998 Sep 18;435(2-3):138-42. doi: 10.1016/s0014-5793(98)01021-7.
p62 is a novel immediate early response gene encoding a ubiquitin chain binding protein. To investigate the mechanism of p62 gene expression, we isolated and characterized the 20 kb long human p62 gene. The p62 gene contains seven introns and eight exons. The splice sites conformed to the GT/AG rule, except introns 6 and 7 which used the unusual GC dinucleotides. The p62 promoter is TATA-less, and 357 nucleotides of the 5'-flanking region contain basic machineries for transcription. A reporter gene linked to 1800 nucleotides of the 5'-flanking region was rapidly activated by various extracellular signals. The presence of a CpG island as well as multiple binding sites for SP-1, AP-1, NF-kappaB, and Ets-1 family in the promoter region supports the regulated activation of the p62 gene.
p62是一种编码泛素链结合蛋白的新型即刻早期反应基因。为了研究p62基因表达的机制，我们分离并鉴定了长达20 kb的人类p62基因。p62基因包含7个内含子和8个外显子。剪接位点符合GT/AG规则，但内含子6和7使用了不寻常的GC二核苷酸。p62启动子无TATA盒，5'侧翼区域的357个核苷酸包含转录的基本机制。与5'侧翼区域1800个核苷酸相连的报告基因可被各种细胞外信号快速激活。启动子区域存在一个CpG岛以及多个SP-1、AP-1、NF-κB和Ets-1家族的结合位点，这支持了p62基因的调控激活。
