Lee Y H, Ko J, Joung I, Kim J H, Shin J
Division of Tumor Virology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
FEBS Lett. 1998 Nov 6;438(3):297-300. doi: 10.1016/s0014-5793(98)01323-4.
p62 is a cytoplasmic ubiquitin chain binding protein. Upon a variety of extracellular signals, both transcript and protein levels of p62 were rapidly increased. These signals include phorbol 12-myristate 13-acetate (PMA) and calcium ionomycin for peripheral blood mononuclear cells, serum or PDGF for serum-starved NIH3T3 cells, IL-3 for the G1 arrested pre-B cell line Ba/F3, and PMA for a human promyelocyte line U937. The elevation of p62 transcript level is due to temporal stabilization of mRNA and rapid activation of the p62 gene. Cycloheximide-induced enhanced transcription suggests the immediate early response of the p62 gene. The rapid induction of p62 indicates the presence of a novel ubiquitination-mediated regulatory mechanism during cell proliferation and differentiation.
p62是一种细胞质泛素链结合蛋白。在多种细胞外信号作用下，p62的转录水平和蛋白水平均迅速升高。这些信号包括用于外周血单核细胞的佛波酯12 - 肉豆蔻酸酯13 - 乙酸酯（PMA）和钙离子载体，用于血清饥饿的NIH3T3细胞的血清或血小板衍生生长因子（PDGF），用于G1期停滞的前B细胞系Ba/F3的白细胞介素-3（IL-3），以及用于人早幼粒细胞系U937的PMA。p62转录水平的升高是由于mRNA的暂时稳定和p62基因的快速激活。放线菌酮诱导的转录增强表明p62基因的即时早期反应。p62的快速诱导表明在细胞增殖和分化过程中存在一种新型的泛素化介导的调节机制。
