Segura A, Moreno M, Molina A, García-Olmedo F
Departamento de Biotecnología-UPM, ETS Ingenieros Agrónomos, Madrid, Spain.
FEBS Lett. 1998 Sep 18;435(2-3):159-62. doi: 10.1016/s0014-5793(98)01060-6.
Antimicrobial peptides (So-D1-7) were isolated from a crude cell wall preparation from spinach leaves (Spinacia oleracea cv. Matador) and, judged from their amino acid sequences, six of them (So-D2-7) represented a novel structural subfamily of plant defensins (group IV). Group-IV defensins were also functionally distinct from those of groups I-III. They were active at concentrations < 20 microM against Gram-positive (Clavibacter michiganensis) and Gram-negative (Ralstonia solanacearum) bacterial pathogens, as well as against fungi, such as Fusarium culmorum, F. solani, Bipolaris maydis, and Colletotrichum lagenarium. Fungal inhibition occurred without hyphal branching. Group-IV defensins were preferentially distributed in the epidermal cell layer of leaves and in the subepidermal region of stems.
抗菌肽（So-D1-7）是从菠菜叶（Spinacia oleracea cv. Matador）的粗细胞壁制剂中分离出来的。根据其氨基酸序列判断，其中六种（So-D2-7）代表了植物防御素的一个新结构亚家族（IV组）。IV组防御素在功能上也与I-III组不同。它们在浓度<20 microM时对革兰氏阳性菌（密歇根棒杆菌）和革兰氏阴性菌（茄科劳尔氏菌）病原体具有活性，同时也对真菌具有活性，如禾谷镰刀菌、茄腐镰刀菌、玉米小斑病菌和葫芦炭疽病菌。真菌抑制作用在没有菌丝分支的情况下发生。IV组防御素优先分布在叶片的表皮细胞层和茎的表皮下区域。
