Rosner A, Maslenin L, Spiegel S
Department of Virology, ARO, The Volcani Center, Bet Dagan, Israel.
J Virol Methods. 1998 Sep;74(1):109-15. doi: 10.1016/s0166-0934(98)00076-7.
A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates.
一种基于聚合酶链反应（PCR）产物所产生的RNA转录本电泳迁移率差异的方法，被用于病毒分离株的鉴别。通过PCR将T7 RNA聚合酶启动子连接到扩增的李坏死环斑病毒（PNRSV）序列上。然后，PCR产物用作转录模板。源自不同PNRSV分离株的单链转录本在聚丙烯酰胺凝胶中的电泳迁移率有所不同，这可能是由于转录本构象多态性（TCP）所致。该程序被应用于PNRSV分离株的鉴别。
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