Yano T, Yano Y, Horikawa S, Ozasa H, Okada S, Otani S, Hagiwara K
Division of Applied Food Research, The National Institute of Health and Nutrition, Tokyo, Japan.
Biochem Pharmacol. 1998 Aug 15;56(4):543-6. doi: 10.1016/s0006-2952(98)00092-6.
In this study, we investigated some factors contributing to renal regeneration after acute renal failure (ARF) induced by vitamin E (VE) deficiency and glutathione (GSH) depletion. Acute renal failure was induced by feeding rats a vitamin E-deficient diet for 6 weeks and then injecting buthionine sulfoximine (BSO), a glutathione-depleting agent. The level of hepatocyte growth factor (HGF), a renotropic factor for regeneration in the kidney, showed a transient increase at 5 hr after the BSO treatment. Subsequently, renal ornithine decarboxylase (ODC) activity, a marker of G1 phase, and labeling index (LI) of proliferating cell nuclear antigen (PCNA), a marker of DNA synthesis (S phase), reached peaks at 10 and 53 hr after the injection, respectively. Thus, it appears that the increase in ornithine decarboxylase activity and subsequent elevation in proliferating cell nuclear antigen labeling index following the increase in the hepatocyte growth factor level in the kidneys are closely related to the renal regenerative response after acute renal failure.
在本研究中，我们调查了一些导致维生素E（VE）缺乏和谷胱甘肽（GSH）耗竭诱发急性肾衰竭（ARF）后肾脏再生的因素。通过给大鼠喂食维生素E缺乏饮食6周，然后注射谷胱甘肽耗竭剂丁硫氨酸亚砜亚胺（BSO）来诱发急性肾衰竭。肝细胞生长因子（HGF）是一种促进肾脏再生的促肾因子，在BSO治疗后5小时其水平出现短暂升高。随后，作为G1期标志物的肾脏鸟氨酸脱羧酶（ODC）活性以及作为DNA合成（S期）标志物的增殖细胞核抗原（PCNA）标记指数（LI）分别在注射后10小时和53小时达到峰值。因此，肾脏中肝细胞生长因子水平升高后鸟氨酸脱羧酶活性的增加以及随后增殖细胞核抗原标记指数的升高似乎与急性肾衰竭后的肾脏再生反应密切相关。
