Galcheva-Gargova Z, Gangwani L, Konstantinov K N, Mikrut M, Theroux S J, Enoch T, Davis R J
Program in Molecular Medicine, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA.
Mol Biol Cell. 1998 Oct;9(10):2963-71. doi: 10.1091/mbc.9.10.2963.
The zinc finger protein ZPR1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. The function of nuclear ZPR1 has not been defined. Here we demonstrate that ZPR1 accumulates in the nucleolus of proliferating cells. The role of ZPR1 was examined using a gene disruption strategy. Cells lacking ZPR1 are not viable. Biochemical analysis demonstrated that the loss of ZPR1 caused disruption of nucleolar function, including preribosomal RNA expression. These data establish ZPR1 as an essential protein that is required for normal nucleolar function in proliferating cells.
用有丝分裂原处理细胞后,锌指蛋白ZPR1从细胞质转移至细胞核。细胞核中ZPR1的功能尚未明确。在此我们证明,ZPR1在增殖细胞的核仁中积累。我们使用基因敲除策略研究了ZPR1的作用。缺乏ZPR1的细胞无法存活。生化分析表明,ZPR1的缺失导致核仁功能紊乱,包括前核糖体RNA的表达。这些数据表明,ZPR1是增殖细胞正常核仁功能所必需的一种重要蛋白质。